sequencing results  (Bioedit Company)

Journal: ASM Case Reports

Article Title: Mycobacterium wolinskyi as an emerging cause of pacemaker pocket infection and lead endocarditis: a case report and genomic characterization

doi: 10.1128/asmcr.00146-25

Figure Lengend Snippet: Phylogenetic analysis based on 16S rRNA ( A ), hsp65 ( B ) , rpoB ( C ), and recA ( D ) sequences for several fast-growing Mycobacteria . For the 16S rRNA gene, the corresponding near full-length nucleotide sequence (1,388 bp) was used, while for the hsp65 (372 bp) , rpoB (723 bp), and recA (951 bp) genes, a partial sequence of identical length was used for the phylogenetic analysis, which was performed via the Geneious Tree Builder using the Neighbor-Joining tree build method and the Tamura-Nei Genetic Distance model (Geneious Prime GraphPad software). In cases where a particular strain harbored multiple 16S rRNA copies, these are indicated by (1) and (2).

Article Snippet: Because of discrepant 16S rRNA sequencing results and low ANI percentage compared with the M. wolinskyi reference genome (strain ATCC 700010), a phylogenetic analysis was performed.

Techniques: Sequencing, Software

Journal: BMC Genomics

Article Title: Assembly and comparative analysis of the complete mitochondrial genome of two species of Argentina (Rosaceae)

doi: 10.1186/s12864-025-12442-8

Figure Lengend Snippet: Validation of mitochondrial RNA editing sites in A. anserina . A Cloning of mitochondrial RNA editing sites in A. anserina . B Sequencing chromatograms of mitochondrial RNA editing sites in A. anserina

Article Snippet: Amplicons of correct size were Sanger sequenced by Sangon Biotech, and sequencing results were aligned and visualized using SeqMan [ ] and Snapgene software (GSL Biotech LLC, Chicago, IL, USA).

Techniques: Biomarker Discovery, Cloning, Sequencing

Journal: BMC Genomics

Article Title: Identification of a complex chromosomal insertion using the chromosome conformation based karyotyping technique for the implementation of PGT-SR

doi: 10.1186/s12864-025-12515-8

Figure Lengend Snippet: Workflow of the C-MoKa Technology. This schematic illustrates the streamlined workflow for detecting chromosomal translocations. Isolated PBMCs are formaldehyde-fixed to preserve nuclear structures. Genomic DNA is then fragmented by DNase I digestion, followed by end labeling and ligation. After crosslink reversal and purification, DNA is sheared to optimal size for library construction, which includes end repair, adapter ligation, and PCR amplification. Finally, libraries are sequenced on a high-throughput platform, and bioinformatic analysis identifies translocated chromosomes

Article Snippet: The high-throughput sequencing results of 20 biopsies of human early blastocyst trophoblast cells analyzed during this study are publicly available in the National Center for Biotechnology Information BioSample database under accession number PRJNA128600 ( https://www.ncbi.nlm.nih.gov/sra/PRJNA1286004 ).

Techniques: Isolation, End Labeling, Ligation, Purification, Adapter Ligation, Amplification, High Throughput Screening Assay